NAD+ · Analytical Methods
Analytical Methods
Characterizing NAD+ means answering two analytical questions: is this the correct molecule, and how pure is it? This reference describes the standard methods used (chromatography and mass spectrometry) and what each establishes. It is an analytical-chemistry reference for laboratory work. For research use only. Not for human consumption, diagnostic, or therapeutic use.
HPLC: purity and separation
High-performance liquid chromatography passes the dissolved sample through a column that separates it into its constituent species by their interaction with the stationary phase. The resulting chromatogram lets analysts quantify the target compound against impurities, producing the ≥98% (HPLC verified) purity figure documented for NAD+.
- Purity by
- HPLC
- Identity by
- LC-MS / mass spectrometry
- Expected mass
- 663.43 g/mol
Mass spectrometry for identity
Liquid chromatography–mass spectrometry (LC-MS) measures the mass of the separated species, which confirms the compound's identity by molecular-weight confirmation against the expected formula. For NAD+, the expected molecular weight is 663.43 g/mol.
Mass confirmation is what distinguishes NAD+ from closely related analogs that might otherwise look similar by chromatography alone.
Identity confirmation in practice
For NAD+, identity rests on matching the measured mass and, where relevant, spectroscopic signatures to the expected structure.
The outcome of this characterization is summarized on the certificate of analysis. For research use only. Not for human consumption, diagnostic, or therapeutic use.
Available from Blueprint Labs
NAD+ 100mg
Third-party tested, NAD+ supplied as a research material with a certificate of analysis available.
Questions, answered
How is NAD+ analyzed?
NAD+ is characterized by HPLC (purity) and LC-MS (identity). These are the standard methods reflected on its certificate of analysis.
How is the identity of NAD+ confirmed?
By mass spectrometry. The measured mass is checked against the expected molecular weight (663.43 g/mol).
What does HPLC tell you about NAD+?
HPLC separates the sample so the target compound can be quantified against impurities, giving the ≥98% (HPLC verified) purity figure.
Are these analytical results a use approval?
No. They document analytical quality for research materials only. For research use only. Not for human consumption, diagnostic, or therapeutic use.