TB-500 · Analytical Methods
Analytical Methods
Characterizing TB-500 means answering two analytical questions: is this the correct molecule, and how pure is it? This reference describes the standard methods used (chromatography and mass spectrometry) and what each establishes. It is an analytical-chemistry reference for laboratory work. For research use only. Not for human consumption, diagnostic, or therapeutic use.
HPLC: purity and separation
High-performance liquid chromatography passes the dissolved sample through a column that separates it into its constituent species by their interaction with the stationary phase. The resulting chromatogram lets analysts quantify the target compound against impurities, producing the ≥98% (HPLC + LC-MS verified) purity figure documented for TB-500.
- Purity by
- HPLC
- Identity by
- LC-MS / mass spectrometry
- Expected mass
- 4963.5 g/mol (full Tβ4)
Mass spectrometry for identity
Liquid chromatography–mass spectrometry (LC-MS) measures the mass of the separated species, which confirms the compound's identity by sequence-consistent mass confirmation (and, for peptides, sequence analysis). For TB-500, the expected molecular weight is 4963.5 g/mol (full Tβ4).
Mass confirmation is what distinguishes TB-500 from closely related analogs that might otherwise look similar by chromatography alone.
Identity confirmation in practice
Because TB-500 is defined by its amino-acid sequence (Ac-Ser-Asp-Lys-Pro (SDKP active motif; full 43-residue Tβ4)), identity work checks that the measured mass is consistent with that sequence. Deviations flag synthesis or degradation issues.
The outcome of this characterization is summarized on the certificate of analysis. For research use only. Not for human consumption, diagnostic, or therapeutic use.
Available from Blueprint Labs
Wolverine (bpc 5mg, tb500 5mg)
Third-party tested, TB-500 supplied as a research material with a certificate of analysis available.
Questions, answered
How is TB-500 analyzed?
TB-500 is characterized by HPLC (purity) and LC-MS (identity). These are the standard methods reflected on its certificate of analysis.
How is the identity of TB-500 confirmed?
By mass spectrometry. The measured mass is checked against the expected molecular weight (4963.5 g/mol (full Tβ4)) and sequence.
What does HPLC tell you about TB-500?
HPLC separates the sample so the target compound can be quantified against impurities, giving the ≥98% (HPLC + LC-MS verified) purity figure.
Are these analytical results a use approval?
No. They document analytical quality for research materials only. For research use only. Not for human consumption, diagnostic, or therapeutic use.