Semax · Analytical Methods
Analytical Methods
Characterizing Semax means answering two analytical questions: is this the correct molecule, and how pure is it? This reference describes the standard methods used (chromatography and mass spectrometry) and what each establishes. It is an analytical-chemistry reference for laboratory work. For research use only. Not for human consumption, diagnostic, or therapeutic use.
HPLC: purity and separation
High-performance liquid chromatography passes the dissolved sample through a column that separates it into its constituent species by their interaction with the stationary phase. The resulting chromatogram lets analysts quantify the target compound against impurities, producing the ≥99% (HPLC + LC-MS verified) purity figure documented for Semax.
- Purity by
- HPLC
- Identity by
- LC-MS / mass spectrometry
- Expected mass
- 813.92 g/mol
Mass spectrometry for identity
Liquid chromatography–mass spectrometry (LC-MS) measures the mass of the separated species, which confirms the compound's identity by sequence-consistent mass confirmation (and, for peptides, sequence analysis). For Semax, the expected molecular weight is 813.92 g/mol.
Mass confirmation is what distinguishes Semax from closely related analogs that might otherwise look similar by chromatography alone.
Identity confirmation in practice
Because Semax is defined by its amino-acid sequence (Met-Glu-His-Phe-Pro-Gly-Pro (MEHFPGP)), identity work checks that the measured mass is consistent with that sequence. Deviations flag synthesis or degradation issues.
The outcome of this characterization is summarized on the certificate of analysis. For research use only. Not for human consumption, diagnostic, or therapeutic use.
Available from Blueprint Labs
Semax
Third-party tested, Semax supplied as a research material with a certificate of analysis available.
Questions, answered
How is Semax analyzed?
Semax is characterized by HPLC (purity) and LC-MS (identity). These are the standard methods reflected on its certificate of analysis.
How is the identity of Semax confirmed?
By mass spectrometry. The measured mass is checked against the expected molecular weight (813.92 g/mol) and sequence.
What does HPLC tell you about Semax?
HPLC separates the sample so the target compound can be quantified against impurities, giving the ≥99% (HPLC + LC-MS verified) purity figure.
Are these analytical results a use approval?
No. They document analytical quality for research materials only. For research use only. Not for human consumption, diagnostic, or therapeutic use.